Merck, 234138-1MG, Collagen, Type I, Human Skin

General description
Collagen type 1 is a fibrillar collagen present abundantly in skin, bone, vasculature, and calcified tissues of teeth and bone. It is also seen in organs like the heart and lungs. Collagen type 1 is a heterotrimer consisting of two α1 (CIα1) and one α2 (CIα2) chains with a helical region made of repeating units of proline and hydroxyproline.
Application
Collagen Type I, Human Skin has been used as a skin protein to study the effect of age-related NADH oxidase (ar-NOX)-catalyzed oxidation on skin proteins. It has also been used as a control sample in High-resolution mass spectrometry (HRMS) analysis.
Biochem/physiol Actions
Collagen type 1 is a main component of the dermal extracellular matrix (ECM) and participates in skin elasticity. Mutations in collagen type 1 are associated with osteogenesis imperfecta and Ehlers–Danlos syndrome.
Physical form
Salt-free.
Preparation Note
Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
The recommended concentration is 0.01 to 0.5 M Acetic Acid (or another acidic buffer), with a maximum concentration of 5 mg/ml. The product has been tested at 0.5 M Acetic Acid and is stable for 1 month at 2-8°C. After reconstitution, aliquot and freeze at -20°C. Stock solutions remain stable for up to 6 months at this temperature.
Other Notes
Gallop, P.M., and Siefert, S. 1963. Methods Enzymol. 6, 635.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Toxicity: Standard Handling (A)