Merck, 236600-1MG, C-Reactive Protein, Human Ascites

General description
M.W. 23,000 (monomer); 115,000 (pentamer).
Native C-reactive protein from human ascites. CRP is a major acute-phase plasma protein that is dramatically elevated in patients with acute-phase conditions. Composed of five identical noncovalently linked subunits. Exhibits Ca²⁺-dependent binding to a variety of substrates. Important in diagnosis of rheumatoid arthritis. Suitable for immunological studies. In purified states and in plasma the monomeric form (M.W. ~21 kDa) aggregates as a cyclic pentamer.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 150 mM NaCl, 20 mM Tris, 2 mM CaCl₂, 0.1% NaN₃, pH 7.5.
Preparation Note
Prepared from ascites that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Analysis Note
Single band by SDS-PAGE
Other Notes
Christner, R.B., and Mortensen, R.F. 1994. J. Biol. Chem. 269, 9760.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)