Description
General description
Test Principle With CHEMICON®′s BrdU Cell Proliferation Assay Kit, mouse monoclonal antibody is used to detect the BrdU in a sample. After addition of goat anti-mouse IgG-peroxidase conjugated secondary antibody, substrate and stop solution, the amount of BrdU is determined. The higher the OD, the higher the BrdU concentration in the sample.
Application
The BrdU Cell Proliferation Assay Kit is used to detect the BrdU in a sample.
The CHEMICON® BrdU Cell Proliferation Assay Kit is a non-isotopic assay for the in vitro quantitative detection of newly synthesized DNA of actively proliferating cells. For Research Use Only. Not for use in diagnostic procedures.
Packaging
200 assays
Preparation Note
Maintain the kit at 2° to 8°C under sterile conditions for up to 6 months after date of receipt.
Before first use: remove the Fixative Solution and place at room temperature for at least 4 hours prior to use. Precipitates that may occur during cold storage should go back into solution.
Other Notes
BrdU Reagent: (Part No. 2750a) One 15 μL vial of a 500X solution of BrdU.
Fixing Solution: (Part No. 2750b) One 40 mL bottle.
Prediluted BrdU Detection Antibody: (Part No. 2750c) One 20 mL vial of an anti-BrdU solution.
Stop Solution: (Part No. 2750d): One 25 mL bottle of 2.5N sulfuric acid solution.
Goat anti-Mouse IgG, Peroxidase labeled: (Part No. 2750e) One 15 μL vial of a 2000X solution.
Conjugate Diluent: (Part No. 2750f) One 25 mL bottle.
Substrate: (Part No. 2750g) One 25 mL bottle, ready to use TMB.
50X Plate Wash Concentrate: (Part No. 2750h) One 90 mL bottle of buffered Tris with surfactant.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
