Description
General description
Caged Ca²⁺ chelator that undergoes a major and rapid decrease in Ca²⁺-binding affinity upon photolysis. Can be microinjected or used with permeabilized cells. May also be useful as caged Mg²⁺ chelator. Kd (Ca²⁺) = 5 nM; Kd(Mg²⁺) = 2-5 µM.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Mulkey, R.M., and Zucker, R.S. 1993. J. Physiol. 462, 243.
Zucker, R.S. 1993. Cell Calcium 14, 87.
McCray, J.A., et al. 1992. Biochemistry31, 8856.
Delaney, K.R., and Zucker, R.S. 1990. J. Physiol.426, 473.
Kaplan, J.H., and Ellis-Davies, G.C. 1988. Proc. Natl. Acad. Sci. USA85, 6571.
Morad, M., et al. 1988. Science241, 842.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)