Description
General description
CRM₁₉₇ is a nontoxic diphtheria toxin that has a glycine to glutamic acid mutation at amino acid 52 in fragment A, resulting in the complete loss of enzymatic activity. Does not catalyze the ADP ribosylation of EF-2 factor. Immunologically, the CRM₁₉₇ protein is identical to the wild-type toxin.
Cross-reactive material of diphtheria toxin (CRM₁₉₇). A nontoxic diphtheria toxin that has a glycine to glutamic acid mutation at amino acid 52 in fragment A that results in complete loss of enzymatic activity. Inhibits smooth muscle cell proliferation in a dose-dependent manner. Immunologically, the CRM₁₉₇ is identical to the wild-type toxin and is useful as a carrier protein of conjugate vaccines.
Biochem/physiol Actions
Cell permeable: no
Primary Target
Mutant with loss of enzymatic activity to catalyze the ADP ribosylation of EF-2 factor
Reversible: no
Physical form
Lyophilized from 10 mM sodium phosphate buffer, 5% lactose, pH 7.4.
Preparation Note
Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 1 year at -70°C.
Please refer to vial label for lot-specific reconstitution volume.
Analysis Note
One major band by SDS-PAGE
Other Notes
Kusano, I., et al. 2001. Cell Struct. Funct.26, 279.
Kaefer, M., et al. 2000. J. Urol.163, 580.
Gupta, R.K., et al. 1997. Vaccine15, 1341.
Bixler, G.S., et al. 1989. Adv. Exp. Med. Biol.251, 175.
Giannini, G., et al. 1984. Nucleic Acids Res.12, 4063.
Uchida, T., et al. 1973. J. Biol. Chem. 248, 3851.
Pappenheimer, Jr., A.M., et al. 1972. Immunochem. 9, 891.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
