Description
General description
A cell-permeable phthalimide compound that is reported to inhibit dynamin GTPase activity (IC₅₀ = 17.4 and 63 µM using purified sheep brain dynamin I and recombinant dynamin II, respectively) in a GTP-competitive manner. Shown to block K⁺-induced SVE (synaptic vesicle endocytosis) of FM4-64 dye into rat brain synaptosomes (IC₅₀ = 12.9 µM), while being ineffective at 30 µM against RME (receptor-mediated endocytosis) of transferrin into human osteosarcoma epithelial U2OS cells, presumably due to poor dynamin II inhibitory potency and/or rapid efflux and/or limited permeability in this cell line.
Packaging
Packaged under inert gas
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Other Notes
Odell, L.R., et al. 2010. J. Med. Chem.53, 5267.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)