Merck, 324717-200MIU, Endoglycosidase H, Streptomyces plicatus, Recombinant, E. coli

General description
Note: 1 mU = 1 milliunit.
Recombinant, streptomyces plicatus endoglycosidase H expressed in E. coli. Catalyzes the hydrolysis of asparagine-linked oligomannose and hybrid, but not complex oligosaccharides from glycoprotein. Endo H cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide.
Physical form
In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.
Other Notes
One unit is defined as the amount of enzyme that will catalyze the release of N-linked oligosaccharides from 1.0 µmol denatured ribonuclease B per min at 37°C, pH 5.5. Cleavage is monitored by SDS-PAGE.
Rao, V., et al. 1995. Structure 3, 449.
Trimble, R.B., et al. 1987. Methods Enzymol. 138, 763.
Trumbly, R.J., et al. 1985. J. Biol. Chem.260, 5683.
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Toxicity: Standard Handling (A)