Merck, 324904-50UG, Epiregulin, Mouse, Recombinant

General description
Recombinant, mouse epiregulin expressed in E. coli with an N-terminal methionine. Epiregulin is shown to inhibit epithelial tumor growth, able to stimulate growth of fibroblasts and other cell types, and has been shown to be an autocrine growth factor in human epidermal keratinocytes.
Biochem/physiol Actions
ED₅₀ = 0.3-1 ng/ml as measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts.
Physical form
Lyophilized from 0.2 µm sterile-filtered PBS, BSA.
Preparation Note
Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Reconstitute to 10 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.
Other Notes
Shirakata, Y., et al. 2000. J. Biol. Chem.275, 5748.
Shelly, M., et al. 1998. J. Biol. Chem.273, 10496.
Das, S.K., et al. 1997. Dev. Biol.190, 178.
Komurasaki, T., et al. 1997. Oncogene15, 2841.
Toyoda, H., et al. 1995. J. Biol. Chem.270, 7495.
Toyoda, H., et al. 1995. FEBS Lett.377, 403.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)