Description
General description
The PI3-Kinase HTRF Assay is a high-performance assay kit that provides a universal method for assaying all Class I PI3-Kinases in a homogeneous 384-well format.
Application
PI 3-Kinase HTRF® Assay; 1920 wells have been used to measure the activity of phosphoinositide-3-kinases (PI3Ks). It has also been used in a biochemical assay to measure the potency and selectivity of LL-00071210 (a selective PI3Kδ inhibitor).
Biochem/physiol Actions
The PI 3-Kinases catalyze the phosphorylation of the three positions of phosphatidylinositols. The Class I enzymes convert phosphatidylinositol 4,5-bisphosphate (PIP2) to phosphatidylinositol 3,4,5-trisphosphate (PIP3) in the presence of ATP and Mg²⁺.
Features and Benefits
Large Stokes shift and time-resolved elements of technology help to reduce compound interference, minimizing concern of false negatives
Picomole sensitivity of the HTRF assay platform enables the integrity of enzyme kinetics to be retained, ensuring the most physiologically relevant assay parameters.
Compatible with all Class I phosphoinositide 3-kinase (PI3K) isoforms: enables relative data comparisons and eliminates the need for multiple assay types.
Reliable, proven assay—based on the technology used in the Millipore, Upstate® KinaseProfiler Service.
Z` factor routinely exceeds 0.8, ensuring consistent, reliable data.
Packaging
Kit capacity: 1920 wells
Other Notes
4X Reaction Buffer
PIP2 (Substrate; 1mM stock)
Stop Reagent A
Stop Reagent B
DM A Detection Reagent
DM B Detection Reagent
DM C Detection Reagent
Legal Information
HTRF is a registered trademark of Cis Bio International
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
