Merck, 344145-100UG, FPTase, Rat, Recombinant, E. coli

General description
M.W. α-subunit: ~48,000; β-subunit: ~46,000
Recombinant, rat FPTase expressed in E. coli. A heterodimeric enzyme that catalyzes the transfer of a 15-carbon isoprenoid group to a C-terminal cysteine in a variety of cellular proteins, including Ras. Recognizes the CaaX motif where the farnesylated cysteine (C) is followed by two aliphatic amino acids (aa) and either leucine or serine (X). Expressed in E. coli as an untagged recombinant protein.
Biochem/physiol Actions
1 pmol of FPTase will transfer 1 pmol of farnesyl to H-Ras in 15 min at 37°C.
Physical form
In 25 mM HEPES, pH 7.2, 40 mM NaCl, 5µM ZnCl2, and 1mM TECP
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Hightower, K.E., et al. 2001. Biochem. J.360, 625.
Zimmerman, K.K., et al. 1998. Protein Expr. Purif.14, 395.
Reiss, Y., et al. 1990. Cell62, 81.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)