Merck, 344146-100UG, FPTase, GST-Fusion, His•Tag®, Rat, Recombinant, E. coli

General description
Recombinant, rat FPTase composed of an α- and a β-subunit (74 and 46 kDa, respectively). The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag sequence. Both the GST moiety and the His•Tag sequence can be utilized to facilitate enzyme capturing in prenylation and binding studies or, when desired, can also be cleaved off by TEV protease. Requires Zn²⁺ and Mg²⁺ for optimal activity.
Biochem/physiol Actions
1 pmol FTase will transfer ≥1 pmol farnesyl to H-Ras in 10 min at 37°C, pH 7.2.
Physical form
In 40 mM NaCl, 25 mM HEPES, 5 mM DTT, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Zimmerman, K.K., et al. 1998. Protein Expr. Purif.14, 395.
Reiss, Y., et al. 1990. Cell62, 81.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)