Merck, 344932, Furin, Human, Recombinant

General description
Recombinant, human furin expressed in vaccinia virus (VV:hFUR713t) infected BSC-40 cells. Furin is a membrane-associated, calcium-dependent, serine protease that belongs to the subtilisin-like pro-hormone convertase (PC) family. Members of this family cleave pro-hormones, neuropeptide precursors, some viral proteins, and bacterial toxins. The preparation was subjected to UV irradiation for 5 minutes to inactivate any residual virus.
This preparation was subjected to UV irradiation for 5 min to inactivate any residual vaccinia virus.
Physical form
In 20 mM Bis-Tris buffer, 1 mg/ml BSA, pH 7.0.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Bassi, D.E., et al. 2001. Mol. Carcinog.31, 224.
Bassi, D.E., et al. 2001. Proc. Natl. Acad. Sci. USA98, 10326.
Creemers, J.W., et al. 2001. J. Biol. Chem.276, 4211.
Wang, X., and Pei, D. 2001. J. Biol. Chem.276, 35953.
Molloy, S.S., et al. 1992. J. Biol. Chem.267, 16396.
One unit is defined as the amount of furin that will cleave 1.0 pmol AMC from the substrate pGlu-Arg-Thr-Lys-Arg-AMC per min at 30°C, pH 7.5.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)