Merck, 345797, α1-3,6-Galactosidase, Recombinant, <i>E. coli</i>

General description
Recombinant α1-3,6-galactosidase expressed in E. coli. Catalyzes the hydrolysis of α1,3-linked and α1,6-linked, non-reducing terminal galactose from complex carbohydrates and glycoproteins. Particularly efficient in removing α-linked galactose under conditions where the pH must be neutral or above, as with live cells.
Physical form
In 50 mM sodium phosphate buffer, pH 7.5.
Other Notes
One unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol p-nitrophenol from p-nitrophenyl-α-D-galactopyranoside per min at 25°C, pH 6.5.
Prime, S., et al. 1996. J. Chromatogr. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)