Merck, 345852-50UG, Geranylgeranyltransferase I, GST-Fusion, His•Tag®, Rat, Recombinant, E. coli

General description
Recombinant, rat GGTase I composed of an α- and a β-subunit (74 and 43 kDa, respectively). The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag sequence. Both the GST moiety and the His•Tag sequence can be utilized to facilitate enzyme capturing in prenylation and binding studies or, when desired, can also be cleaved by TEV protease. Requires Zn²⁺ for optimal activity. The enzyme is purified with Zn²⁺ tightly bound, so addition of Zn²⁺ to the reaction mix is not necessary unless further manipulation of the enzyme results in release of the ions.
Biochem/physiol Actions
1 pmol enzyme will transfer ≥3 pmol geranylgeranyl to RhoA in 10 min at 37°C, pH 7.2.
Physical form
In 40 mM NaCl, 25 mM HEPES, 5 mM DTT, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Kalinin, A., et al. 2001. Protein Expr. Purif.22, 84.
Zhang, F.L., et al. 1994. J. Biol. Chem.269, 23465.
Moomaw, J.F., and Casey, P.J., 1992. J. Biol. Chem.267, 17438.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)