Description
General description
A cell-permeable anacardic acid (Cat. No. 172050) analog that inhibits protein SUMO modification (IC₅₀ = 3.0 µM using RanGAP1-C2 as substrate) in an ATP-dependent manner by selectively targeting SUMO-activating enzyme E1 and interfering with E1-SUMO intermediate formation independent of E1 active site cysteine. Both ginkgolic acid and anacardic acid are shown to effectively decrease overall SUMOylation of 293T cellular proteins, including p53, in a dose-dependent manner, while neither compound is effective in affecting overall cellular protein ubiquitination or histone H4K8 acetylation in 293T cultures, although both compounds are shown to inhibit histone lysine acetylation by PCAF in cell-free acetylase assays. Also available as a 25 mM solution in DMSO (Cat. No. 5.08197).
Packaging
Packaged under inert gas
Preparation Note
Following intitial thaw, aliquot and freeze (-20°C).
Other Notes
Fukuda, I., et al. 2009. Chem. Biol.16, 133.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Irritant (B)