Merck, 347420, β-Glucuronidase, <i>Helix pomatia</i>

General description
Native β-glucuronidase from Helix pomatia. Useful for hydrolyzing urinary steroid conjugates prior to steriod assays. Note: 1 MU = 1,000,000 units.
Native β-glucuronidase from Helix pomatia. Useful for hydrolyzing urinary steroid conjugates prior to steriod assays. Note: 1 MU = 1,000,000 units.
Biochem/physiol Actions
β-Glucuronidase, a member of the 2 glycosyl hydrolase family, is involved in the hydrolysis of β-glucuronic acid residues from the non-reducing termini of glycosaminoglycans (GAGs). β-Glucuronidase catalyzes the complete hydrolysis of urinary conjugates. β-Glucuronidase serves as a possible candidate enzyme for gene-mediated enzyme prodrug therapy for glucuronide prodrugs. β-glucuronidase-responsive albumin binding prodrugs might be an effective therapeutic practice for treating solid tumors.
Physical form
A 65% saturated ammonium sulfate suspension.
Preparation Note
Following reconstitution, store in refrigerator (4°C). Stock solutions are stable for up to 3 months at 4°C.
Other Notes
One unit is defined as the amount of enzyme that will liberate 1.0 µg phenolphthalein from phenolphthalein glucuronide per h at 37°C, pH 5.0.
Shibasaki, H., et al. 2001. Steroids66, 795.
Graef, V., et al. 1977. Clin. Chem.23, 532.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)