Merck, 358-05A, Human Internal Thoracic Artery Smooth Muscle Cells: HITASMC, adult

General description
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Human Internal Thoracic Smooth Muscle Cells (HITASMC) are derived from the normal internal thoracic arteries (formerly called internal mammary arteries).HITASMC, along with Human Coronary Artery Smooth Muscle Cells, both from Cell Applications, Inc. have been used to investigate the gene expression differences between smooth muscle cells from different arteries, underlying their differential response to injuries and proliferation stimuli (Lange, 2013).Characterization: positive for smooth muscle cell specific alpha-actin expression.
Application
gene expression, response to injury and proliferation stimuli, blood supply to anterior chest wall and breasts, coronary artery bypass grafting, revascularization
Biochem/physiol Actions
Artery
Preparation Note
2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
Can be cultured at least 8 doublings

Please refer to the HITASMC Culture Protocol.
Other Notes
Basal Medium containing 10% FBS & 10% DMSO
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.