Merck, 371209-10UG, Dkk-2, Mouse, Recombinant

General description
Dkk-2, Mouse, Recombinant, consists of amino acids 26-259 and expressed in S. frugiperda 21 cells with a C-terminal His•Tag sequence.
Recombinant, mouse Dickkopf related protein 2 (Dkk-2) consisting of amino acids 26-259 and expressed in S. frugiperda 21 cells with a C-terminal His•Tag sequence. Dkk proteins are expressed in mesenchymal tissues and control epithelial transformations.
Biochem/physiol Actions
Inhibition assay: Inhibits WNT-3a-induced alkaline phosphatase production in MC3T3-E1 mouse osteoblastic cells in the presence of BMP-2.

Competetive Binding Assay:
Inhibits 50% binding of biotinylated rhDkk-1 (200 ng/ml) to immobilized rmKremen (4 µg/ml) at a concentration of 5-15 µg/ml.
Physical form
Lyophilized from 0.2 µm filtered PBS, BSA.
Preparation Note
Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Reconstitute to 100 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.
Other Notes
Morvan, F. et al. 2006. J. Bone Miner. Res.21, 934.
Mukhopadhyay, M. et al. 2006. Development133, 2149.
Niehrs, C. 2006. Oncogene25, 7469.
Li, X. et al. 2005. Nat. Genet.37, 945.
Bullock, C.M. et al. 2004. Mol. Pharmacol.65, 582.
Mao, B. and C. Niehrs 2003. Gene302, 179.
Brott, B. and S.Y. Sokol 2002. Mol. Cell. Biol.22, 6100.
Li, L. et al. 2002. J. Biol. Chem.277, 5977.
Mao, B. et al. 2002. Nature417, 664.
Mao, B. et al. 2001. Nature411, 321.
Wu, W. et al. 2000. Current Biol.10,1611.
Monaghan, A.P. et al. 1999. Mech. Dev.87, 45.
Krupnik, V.E. et al. 1999. Gene238, 301.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)