Merck, 374817-1GM, Hemocyanin, Keyhole Limpet, Megathura crenulata, Glycerol

General description
Carrier protein that has many available primary amines to facilitate protein conjugation. Used to immunize animals to elicit antibodies to small molecules (haptens) by covalent conjugation. Protein: ≥10 mg/ml. Sold on the basis of protein content.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 10 mM MgSO₄, 5 mM BES, 50% glycerol, 0.9% sodium chloride, pH 6.5.
Preparation Note
Further dilute with PBS or physiological saline. Material can be dialyzed to remove the glycerol or used as is for conjugation with glutaraldehyde, carbodiimide, or mixed anhydrides.KLH is an extremely large protein complex that has a tendency to aggregate and precipitate from solutions. Use a buffer when diluting KLH and maintain the magnesium concentration at ≥10 mM. Store KLH solutions at 4°C. DO NOT freeze, vortex or agitate to dissolve KLH. Freezing or agitating KLH will cause protein aggregation and precipitation.
Other Notes
Hayashi, K., et al. 1992. Virchows Archiv. B Cell Pathol.63, 37.
Shuler, K.R., et al. 1992. J. Immunol. Methods156, 137.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)