Description
General description
Pwo SuperYield DNA Polymerase is originally isolated from Pyrococcus woesei.
The enzyme accepts modified nucleotides for efficient labeling of nucleic acids by PCR.
PCR products are blunt-ended directly useable for blunt-end ligation.
Using the magnesium-containing reaction buffer supplied, the final MgCl₂ concentration is 1.5mM.
The core component of the Pwo Master is the proofreading Pwo SuperYield DNA Polymerase which has both polymerase activity and a 3′–5′ exonuclease (proofreading) activity. Pwo SuperYield DNA Polymerase combines Pwo DNA Polymerase with an optimized buffer system. This buffer system enhances the enzymatic properties of the enzyme, resulting in higher yields of the amplification without changing the fidelity of DNA synthesis.
Application
Use this premixed, double-concentrated formulation containing PwoSuperYield DNA Polymerase, a new optimized buffer system, and PCR grade nucleotides, if your application requires better fidelity than provided by (FastStart) Taq DNA Polymerase. The product can be applied for:
Accurate amplification of DNA critical applications
High-throughput PCR
High fidelity PCR
Site-directed mutagenesis
Cloning
Gene expression
Features and Benefits
Pwo Master is a ready-to-use, 2x concentrated master mix. Pwo SuperYield DNA Polymerase has polymerase and 3′ → 5′ exonuclease proofreading activity, including an optimized buffer. The buffer enhances enzyme performance, synthesizing higher DNA yields of high fidelity.
Higher yield and 18-fold higher fidelity
compared to Taq DNA Polymerase.
Maximize convenience.
Fewer steps are required with the ready-to-use master mix. Simply add template DNA, primers, and PCR-grade water then start the reaction.
Reduce setup time.
Store at +2 to +8°C - no need to thaw reagents.
Packaging
1 kit containing 2 components
Analysis Note
Quality control is performed using human genomic DNA, with primers specific for tPA that generate a 1.7kb product.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Volume Activity: 2.5.U - 50 μl reaction
Legal Information
Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.NOTICE TO PURCHASER: LIMITED LICENSE
Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche. All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany. Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.
