Merck, 382129-500U, HIV Reverse Transcriptase, Recombinant, E. coli

General description
Recombinant HIV reverse transcriptase expressed in E. coli. Catalyzes error-prone synthesis on DNA and RNA templates. Provides an excellent target for evaluating antiviral agents.
Application
HIV Reverse Transcriptase, Recombinant, E.coli has been used as a standard in reverse transcriptase activity assay.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 10 mM potassium phosphate, 1 mM DTT, 20% glycerol, pH 7.4.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
One unit is defined as the amount of enzyme that will incorporate 1.0 nmol of labeled dTTP into acid-insoluble material in 20 min at 37°C, pH 8.3.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)