Merck, 386023-50UG, Anti-Hsp47 Mouse mAb (M16.10A1)

General description
Anti-Hsp47, mouse monoclonal, clone M16.10A1, recognizes the ~47 kDa Hsp47 protein in wide variety of species. It is validated for use in ELISA, EIA, WB, ICC, IF, IP, and IHC (paraffin sections).
Protein G purified mouse monoclonal antibody. Recognizes the ~47 kDa Hsp47 protein.
Recognizes the ~47 kDa Hsp47 protein.
Immunogen
Hsp47 from L6 cells
Rat
Application
ELISA (see comments)
Enzyme Immunoassay (see comments)
Immunoblotting (1 µg/ml, colorimetric)
Immunocytochemistry (1 µg/ml)
Immunofluorescence (5 µg/ml)
Immunoprecipitation (5 µg/ml)
Paraffin Sections (see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In PBS, 50% glycerol, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
3T3 cells, Human breast cancer tissue
Other Notes
Kuroda, K., et al. 1998. J. Invest. Dermatol.111, 1023.
Nandan, D., et al. 1998. Exp. Cell Res.179, 289.
Clark, E.P., et al. 1991. J. Biol. Chem.266, 17230.
Vaillancourt, J.P., and Cates, G.A. 1991. Biochem. J.274, 793.
Cates, G.A., et al. 1987. Biochem. Cell Biol. 65, 767.
Saga, S., et al. 1987. J. Cell Biol.105, 517.
Nagata, K., et al. 1986. J. Biol. Chem. 261, 7531.
This antibody has also been reported to work for ELISA and enzyme immunoassay. Variables associated with assay conditions will dictate the optimal dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)