Merck, 386040-50UG, Anti-Hsp90 Mouse mAb (AC88)

General description
Protein G purified mouse monoclonal antibody. Recognizes the ~90 kDa Hsp90 protein.
Recognizes the ~90 kDa Hsp90 protein.
This Anti-Hsp90 Mouse mAb (AC88) is validated for use in Immunoblotting, Immunoprecipitation, Immunocytochemistry for the detection of Hsp90.
Immunogen
Achyla ambisexualis
Hsp90 isolated from water mold, Achyla ambisexualis
Application
Immunoblotting (1 µg/ml, colorimetric)
Immunoprecipitation (see comments)
Immunocytochemistry (see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 10 mM PBS, 50% glycerol, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
Heat shocked HeLa cells, Heat shocked 3T3 cells, Heat shocked PC-12 cells, Heat shocked CHO-K1, Heat shocked Rat-2 cells
Other Notes
Cross-reacts with glucocorticoid receptor in rodent. By immunoprecipitation, the antibody favors free Hsp90 over complexed Hsp90. The optimal dilution for immunoprecipitation is assay dependent and must be determined for each sample. This antibody has also been reported to work for immunocytochemistry. Variables associated with assay conditions will dictate the optimal dilution.
Harry, J.L. et al. 1990. Development190, 305.
Minoo, P. et al. 1989 J. Cell. Biol.109, 1937.
Riehl, R.M., et al. 1985. Biochemistry 24, 6586.
Sanchez, E.R., et al. 1985. J. Biol. Chem. 260, 12398.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)