Merck, 389561, Hyaluronidase, <i>Streptomyces hyaluronlyticus</i> nov. sp.

General description
Native hyaluronidase from Streptomyces hyaluronlyticus nov. species. Catalyzes the degradation of hyaluronic acid. Produces δ4,5-unsaturated tetra- and hexasaccharides.

Please Note: The appearance of this product can vary from gray cottony fibers to balled up droplets with no change in quality.
Preparation Note
Following reconstitution, store in the refrigerator (4°C). Stock solutions prepared in water are stable for up to 2 weeks at 4°C and stock solutions prepared in 500 mM acetate buffer, pH 6.0, are stable for up to 1 month at 4°C.
Other Notes
Baker, J.R., et al. 2002. Biochem. J.365, 317.
Mogelerskii, M.S. 1964. Fed. Proc. 23, T559.
One TRU (Turbidity Reducing Unit = 1 U) is defined as the amount of enzyme that will hydrolyze 50% of hyaluronic acid, determined by a 50% reduction in turbidity, in 30 min at 60°C pH 6.0.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)