Merck, 400064-50UG, Anti-Glucose Transporter-4 Rabbit pAb

General description
Anti-Glucose Transporter-4, rabbit polyclonal, recognizes the ~40-43 kDa GLUT-4. It is validated for use in ELISA, Western blotting, immunoprecipitation, and immunohistochemistry on frozen sections.
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~40-43 kDa Glut-4 protein.
Recognizes the ~40-43 kDa GLUT-4 protein.
Immunogen
Mouse
a synthetic peptide corresponding to amino acids near the C-terminus of mouse GLUT-4, conjugated to KLH
Application
ELISA (0.5-1 µg/ml)
Frozen Sections (2-20 µg/ml)
Immunoblotting (1-10 µg/ml; see comments)
Immunoprecipitation (1-10 µg/100 µg tissue membrane)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In PBS, 0.1% BSA, pH 7.5.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Does not cross-react with Hep G2-type transporter or other Glut isoforms. Important: Do not heat samples prior to immunoblotting, as heating will result in protein aggregation. Variables associated with assay conditions will dictate the optimal working dilution.
Mueckler, M. 1994. Eur. J. Biochem. 219, 713.
James, D.E., et al. 1989. Nature 338, 83.
James, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 8368.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)