Merck, 402OA-05A, Human Chondrocytes - Osteoarthritis: HC-OA, adult

General description
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HC-OA Human Chondrocytes are derived from human articular cartilage of donors with osteoarthritis (OA). Osteoarthritis is an inflammatory disease characterized by increased degradation of cartilage tissue in the joint due to overproduction of enzymes degrading the extracellular matrix. Despite the initial proliferation and activation of chondrocytes, they are not able to efficiently repair the degrading cartilage. Instead, chondrocytes undergo terminal differentiation and eventually apoptose, leading to mineralization of cartilage in a process resembling bone formation during development. Thus, HC-OA provide a useful model to study changes in chondrocyte biology in response to abnormal environment of the OA joint.

Characterization: Positive for aggrecan after differentiation.

HC-OA have been used to demonstrate that THF-alpha activates transcription of ADAMTS-4, a metalloproteinase implicated in cartilage degradation in OA, via p38-MAPK-dependent mechanism (Xue, 2013).
Application
model for studying chondrocyte biology in an osteoarthritis joint
Biochem/physiol Actions
Cartilage
Preparation Note
1st passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
Can be cultured at least 10 doublings

Please refer to the HC-OA Culture Protocol.
Other Notes
Basal Medium containing 10% FBS & 10% DMSO
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.