Merck, 407241-50UG, Insulin Degrading Enzyme, His•Tag®, Rat, Recombinant, S. frugiperda

General description
Recombinant, rat insulin degrading enzyme fused to a His•Tag sequence and expressed in S. frugiperda insect cells. Insulin degrading enzyme (Insulysin) is a metalloprotease that degrades insulin and a variety of other peptides, including glucagon, insulin-like growth factors I and II, atrial natriuretic peptide, TGF-α, and amyloid peptides. Reported to be inversely correlated with brain Aβ peptide levels in insulysin-deficient mice compared to wild-type mice.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 100 mM potassium phosphate buffer, 20% glycerol, pH 7.3.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Hersh, L.B. 2003. Curr. Pharm. Des.9, 449.
Miller, B.C., et al. 2003. Proc. Natl. Acad. Sci. USA100, 6221.
Song, E.S., et al. 2003. J. Biol. Chem.278, 49789.
Mukherjee, A., and Hersh, L.B. 2002. J. Alzheimer′s Dis.4, 341.
Song, E.S., et al. 2001. J. Biol. Chem.276, 1152.
Becker, A.B., and Roth, R.A. 1995. Methods Enzymol.248, 693.
One unit is defined as the amount of the enzyme that will catalyze the hydrolysis of 1 µmol iodinated insulin per h at 37°C, pH 7.3.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)