Merck, 407707-10T, PhosphoDetect Anti-Insulin Receptor (pTyr1162/1163) Rabbit pAb

General description
Note: 1 T = 1 test. Sufficient for 10 Western mini blots.
Rabbit polyclonal antibody adsorbed with non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the insulin receptor (IR) protein phosphorylated at Tyr¹¹⁶²/¹¹⁶³ and insulin-like growth factor 1 receptor (IGF-1R) protein phosphorylated at Tyr¹¹⁶²/¹¹⁶³.
Recognizes the ~95 kDa β subunit of the insulin receptor protein phosphorylated at Tyr¹¹⁶² and Tyr¹¹⁶³ in insulin receptor transfected cells. Cross-reacts with the insulin-like growth factor-1 receptor phosphorylated at Tyr¹¹³⁵ and Tyr¹¹³⁶.
This PhosphoDetect Anti-Insulin Receptor (pTyr¹¹⁶²/¹¹⁶³) Rabbit pAb is validated for use in ELISA, WB, ICC for the detection of Insulin Receptor (pTyr¹¹⁶²/¹¹⁶³).
Immunogen
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr¹¹⁶²/¹¹⁶³ phosphorylation sites of human insulin receptor
Application
ELISA (see comments)
Immunoblotting (1:1000)
Immunocytochemistry (see comments)
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Bevan, P. 2001. J. Cell Sci.114, 1429.
Ottensmeyer, F.P., et al. 2000. Biochemistry39, 12103.
Virkamaki, A., et al. 1999. J. Clin. Invest.103, 931.
Schmid, E., at al. 1998. FASEB J.12, 863.
Hari, J. and Roth, R.A. 1987. J. Biol. Chem.262, 15341.
Morgan, D.O., et al. 1986. Proc. Natl. Acad. Sci. USA83, 328.
White, M.F. and Kahn, C.R. 1986. The Enzymes17, 247.
Ebina, Y., et al. 1985. Cell40, 747.
Ganguly, S., et al. 1985. Current Topics in Cellular Regulation27, 83.
Grunberger, G., et al. 1984. Science223, 932.
The immunogen sequence is 100% conserved in the mouse and rat insulin recptor protein, but cross-reactivity has not been tested. This antibody has also been reported to work for ELISA and immunocytochemistry. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)