Merck, 407711-5UG, Interleukin-12, Human, Recombinant, S. frugiperda

General description
Recombinant, human interleukin-12 expressed in S. frugiperda insect cells. Pleiotropic cytokine that is produced by monocytes/macrophages, B cells, and connective tissue-type mast cells. Also known as natural killer cell stimulatory factor and cytotoxic lymphocyte maturation factor. A potent inducer of IFN-γ production and T cell differentiation and function.
Biochem/physiol Actions
ED₅₀ = 50-200 pg/ml as measured by its ability to stimulate the proliferation of PHA-activated human lymphoblasts
Physical form
Lyophilized from sterile-filtered PBS, 50 µg BSA/µg IL-12.
Preparation Note
Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 1 month at 4°C. Avoid freeze/thaw cycles of solutions.
Reconstitute to a concentration ≥1 µg/ml with sterile PBS containing at least 0.1% HSA or BSA.
Other Notes
Kang, K., et al. 1996. J. Immunol. 156, 1402.
Riemann, H., et al. 1996. J. Immunol. 156, 1799.
Brunda, M.J., et al. 1994. J. Leukoc. Biol. 55, 280.
Smith, T.J., et al. 1994. Eur. J. Immunol. 24, 822.
Gubler, U., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 4143.
Stern, A.S., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 6808.
Kobayashi, M., et al. 1989. J. Exp. Med. 170, 827.
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Toxicity: Standard Handling (A)