Merck, 437695-100UL, Anti-Lipoic Acid Rabbit pAb

General description
Rabbit polyclonal antibody supplied as diluted serum. Recognizes native lipoic acid and lipoic acid covalently attached to proteins.
Recognizes native lipoic acid. Does not recognize lipoic acid modified by 4-HNE (4-hydroxy-2-noneal) and other lipid peroxidation products.
This Anti-Lipoic Acid Rabbit pAb is validated for use in ELISA, Frozen Sections, Immunoblotting, Paraffin Sections for the detection of Lipoic Acid.
Immunogen
lipoic acid conjugated to KLH
Application
ELISA (1:500-1:1000)
Frozen Sections (1:50-1:500)
Immunoblotting (1:1000-1:10,000)
Paraffin Sections (1:50-1:500)
Physical form
serum diluted in buffer, 50% glycerol, pH 7.5.
Other Notes
Does not bind lipoic acid modified by 4-HNE (4-hydroxy-2-noneal) and other lipid peroxidation products. Useful for assessing whether protein-bound lipoic acids have undergone certain forms of oxidative modification. The presence of protein-bound lipoic acid can be used as a marker for mitochondria. Variables associated with assay conditions will dictate the optimal working dilution.
Sasaki, M., et al. 2000. J. Autoimmun.15, 51.
Heitzer, T., et al. 2001. Free Radic. Biol. Med.31, 53.
Patrick. L. 2000. Altern. Med. Rev.5, 290.
Shi, S.S., et al. 1999. Antioxid. Redox Signal1, 123.
Humphries, K.M. and Szeda, L.I. 1998. Biochemistry37, 15835.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)