Merck, 444287-5UG, MMP-13, Human, Recombinant, Active

General description
Recombinant, human MMP-13. MMP-13 is a collagenase-3 enzyme that degrades a range of extracellular matrix proteins, including collagen types I, II, III, IV, IX, X, and XIV, gelatin, aggrecan, perlecan and fibronectin. It is distinguished from other human collagenases by the fact that it effectively degrades type II collagen. The importance of MMP-13 is further strengthened by reports that it is a biomarker for poor prognosis in many carcinomas and other type of cancers.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 50 mM HEPES, 10 mM CaCl₂, 20% glycerol, 0.005% Brij® 35 Detergent, pH 7.5. APMA-free.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Freije, M.P.S., et al. 1994. J. Biol. Chem.269,16766.
One unit is defined as the amount of APMA-activated enzyme that will hydrolyze 1µmol MCA-Pro-Leu-Lys-Gly-Leu-DPA-Ala-Arg-NH₂ (Cat. No. 03-32-5032) at 37°C, pH 7.5.
Legal Information
Brij is a registered trademark of Croda International PLC
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)