| NACRES | MA.02 |
|---|---|
| PubChem Substance ID | 24868813 |
| UNSPSC Code | 41102904 |
| EC Number | 246-644-8 |
| Beilstein/REAXYS Number | 162395 |
| InChI key | LTVUCOSIZFEASK-MPXCPUAZSA-N |
| InChI | 1S/C10H10O3/c1-10-6-3-2-5(4-6)7(10)8(11)13-9(10)12/h2-3,5-7H,4H2,1H3/t5-,6+,7+,10+/m0/s1 |
| SMILES string | [H][C@]12C3CC(C(C)=C3)[C@@]1([H])C(=O)OC2=O |
| biological source | synthetic |
| vapor density | 6.1 (20 °C, vs air) |
| vapor pressure | 5 mmHg ( 120 °C) |
| assay | ≥95.0% |
| Quality Level | 100 |
| refractive index | n20/D 1.506 (lit.) |
| density | 1.232 g/mL at 25 °C (lit.) |
| application(s) | hematologyhistology |
| storage temp. | room temp |
Answer:The hardener is methylnadic anhydride, a cyclic anhydride used in epoxy embedding resin formulations.
Answer:The material has an assay of at least 95% and is supplied in a 250 mL bottle.
Answer:No. The MNA-to-DDSA balance affects cured block properties and should follow a validated embedding formulation.
Sigma-Aldrich MNA Epoxy Embedding Medium Hardener is methylnadic anhydride, a cyclic anhydride used as a curing component in resin formulations for biological electron microscopy. The material has a specified assay of at least 95% and is supplied in a 250 mL bottle. When blended with a compatible base epoxy resin and accelerator, the MNA fraction influences hardness and other sectioning properties of the resulting specimen block. The resin formulation may also include DDSA, but the balance between the two anhydrides must be selected deliberately rather than substituted on an equal-quantity basis. Carefully measure and mix the ingredients, avoid introducing moisture and ensure the specimen is dehydrated before infiltration. A controlled cure allows production of a solid block for ultramicrotomy and subsequent transmission electron microscopy examination. Handle the uncured hardener with chemical-resistant protection and ventilation, as it is a hazardous laboratory reagent. This item is the MNA hardener only and does not include epoxy embedding resin, DDSA or accelerator.
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