General description
cOmplete, Mini, EDTA-free tablets, inhibit a broad spectrum of serine and cysteine proteases. In contrast to other cOmplete tablets they do not contain EDTA, leaving the stability and the function of metal-dependant proteins unaffected. The affinity purification of Poly-His-tagged fusion proteins using IMAC (Immobilized Metal Affinity Chromatography) is also facilitated (no dialysis necessary).
Due to the optimized composition of the tablets they show excellent inhibition of serine and cysteine proteases, and are well suited for the protection of proteins isolated from animal tissues, plants, yeast, and bacteria. cOmplete, Mini, EDTA-free tablets, contains both irreversible and reversible protease inhibitors. Metallo- and aspartic proteases are not inhibited.
cOmplete Mini, EDTA-free Tablets are identical to cOmplete Mini Tablets, the only difference being that no EDTA or other chelating (e.g. EGTA) agents are included.
Application
cOmplete™, Mini, EDTA-free, is used for the inhibition of serine and cysteine proteases in bacterial, yeast, plant, and animal cell extracts.
cOmplete, Mini, EDTA-free Tablets, are used for the inhibition of proteolytic activity in small volumes (up to 10 ml) in which EDTA may interfere with protein stability (e.g., metal-containing proteins) or subsequent assays. Since EDTA interferes with IMAC (Immobilized Metal Affinity Chromatography), cOmplete, Mini, EDTA-free, is preferentially used in the isolation process of Poly-His-tagged fusion proteins.
If it is necessary to inhibit proteolytic activity in a large volume (up to 50 ml), we recommend to use cOmplete, EDTA-free.
cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail has been used as a component of
nonidet P-40 (NP-40) buffer for the preparation of cell lysate of neuroblastoma cells
tissue lysis buffer for homogenisation and lysis of prefrontal cortex tissues
ice-cold radioimmunoprecipitation assay (RIPA) buffer for the lysis of Chinese hamster ovary (CHO-K1) cells
Biochem/physiol Actions
Mixture of several protease inhibitors. For the inhibition of serine, cysteine, but not metalloproteases.
Features and Benefits
Convenience: Simply push the quick-dissolving tablet through the foil packaging of the EASYpack into your buffer.
Complete protection: Instantly protect your proteins against a broad range of proteases (see Table 1).
Flexible: Protect proteins in extracts from almost any tissue or cell, including animals, plants, yeast, bacteria, or fungi.
Safe: Choose non-toxic inhibitors that pose no risk to you or those around you.
Packaging
30 individually packed cOmplete Protease Inhibitor Cocktail Tablets in foil blisters. Each tablet is sufficient for a volume of 10 ml solution.
Physical form
Tablets in foil blister packs
Preparation Note
Working concentration: 1 tablet per 10 ml extraction solution
Working solution: Handling
Carefully push the tablet through the foil packaging using the base of your thumb (not fingernail) to prevent the breakage of tablets.
Preparation of Working Solution
One cOmplete Mini EDTA-free tablet is sufficient for the inhibition of the proteolytic activity in 10 ml extraction solution. When very high proteolytic activity is present, one tablet should be used for 7 ml extraction buffer. Tablets can be added directly to the extraction medium. Alternatively a stock solution (7 X conc.) can be prepared.
Stock solution (7 X conc.)
Dissolve one cOmplete Mini EDTA-free tablet in 1.5 ml dist. water or in 1.5 ml 100 mM phosphate buffer, pH 7.0.
Storage conditions (working solution): The stock solution can be stored at 2 to 8 °C for 1 to 2 weeks, or at least 12 weeks at -15 to -25 °C.
Soluble in aqueous buffers, or add directly to extraction media. Alternatively, prepare 7x stock solutions in 1.5 ml water or 100 mM phosphate buffer, pH 7.0. Stock solution is stable for 1-2 weeks at 2 to 8 °C, or at least 12 weeks at -15 to -25 °C. Can be used in thiol-containing solutions at 15 to 25 °C.
Store at 2 to 8 °C. (storage at -15 to -25 °C is also possible)
Analysis Note
Performance-tested with pancreas extract.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Legal Information
cOmplete is a trademark of Roche
