Description
General description
A cell-permeable version of NFAT Inhibitor (Cat. No. 480402) that is modified at the N-terminal with an eleven arginine transduction domain and a three glycine linker sequence. Prevents the activation and proliferation of T cells both in vitro ( ~ 43% at 1 µM using mixed lymphocyte cultures) and in vivo (10 mg/kg using C3H/HeN mice). Shown to be effective in providing immunosuppression in a murine islet xenograft model. Unlike Cyclosporin A (Cat. No. 239835) and FK506, 11R-VIVIT does not cause side effects outside the immune system as a result of calcineurin (Cat. No. 539568) inhibition and therefore serves as a less toxic alternative for in vivo applications. The functional half-life of 11R-VIVIT has been estimated to be ~30 h in Jurkat cells.
Application
Use at 1 µM for preventing the activation and proliferation of T cells
Biochem/physiol Actions
Primary Target
NFAT
Physical form
Supplied as a trifluoroacetate salt.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
H-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Gly-Gly-Gly-Met-Ala-Gly-Pro-His-Pro-Val-Ile-Val-Ile-Thr-Gly-Pro-His-Glu-Glu-OH
Noguchi, H., et al. 2004. Nat. Med.10, 305.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Harmful (C)
