Merck, 50631-U, Ascentis® Express F5, 5 μm HPLC Column

Name: Ascentis® Express F5, 5 μm HPLC Column
Brand: Merck
SKU: 50631-U

Size:1 ea, Core-shell (SPP) Fused Core PFP HPLC column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

Catalog Number: 50631-U

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Category

Reversed-Phase HPLC Columns

Pack Size1 ea

TagsHPLC Columns Analytical Chemistry Analytical Chromatography

CollectionsMerck Analytical Chromatography Products & Lab Supplies Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Ascentis® Express 5 μm F5 is a high-speed, high-performance liquid chromatography column based on the highly efficient Fused-Core® particle design. The bonded, endcapped, dimethylpentafluorophenyl-propylsilane stationary phase of Ascentis Express 5 μm F5 provides a stable, reversed-phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines. In addition to forming π-π and mildly steric interactions, F5 phases also retain compounds by polar interactions. As a result of having both polar and non-polar character, F5 phases can show dual-mode retention behavior, sometimes producing a “U-shaped” retention as a function of acetonitrile content of the mobile phase, with retention increasing at both low and high concentrations of ACN (reversed-phase and HILIC retention modes). Ascentis Express 5 μm F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.
Application

Liquid chromatographic determination of lumacaftor in the presence of ivacaftor and identification of five novel degradation products using high-performance liquid chromatography ion trap time-of-flight mass spectrometry.: This study employed the Ascentis® Express F5, 5 µm HPLC column to determine lumacaftor and its degradation products in the presence of ivacaftor. The application of high-performance liquid chromatography ion trap time-of-flight mass spectrometry provided high sensitivity and resolution, showcasing the column′s efficacy in complex sample matrices (Özcan et al., 2023).

Stability-indicating LC-MS/MS and LC-DAD methods for robust determination of tasimelteon and high resolution mass spectrometric identification of a novel degradation product.: This research highlights the use of the Ascentis® Express F5, 5 µm HPLC column in developing stability-indicating methods for tasimelteon. The column′s performance in both LC-MS/MS and LC-DAD methods ensured accurate and reliable identification of degradation products (Özcan et al., 2020).

Recent trends in ultra-fast HPLC: new generation superficially porous silica columns.: This review discusses advancements in HPLC technology, including the Ascentis® Express F5, 5 µm HPLC column. The article emphasizes the column′s capability in ultra-fast separations, providing enhanced efficiency and resolution in various analytical applications (Ali et al., 2012).

Other Notes
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Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

Specifications

General

UNSPSC Code	41115700
eCl@ss	32110501
Web Name Suffix Override	L × I.D. 15 cm × 4.6 mm, HPLC Column
Web Name Override	Ascentis® Express 90 Å F5 (5 μm) HPLC Columns
NACRES	SB.52
material	stainless steel column
agency	suitable for USP L43
product line	Ascentis®
feature	endcapped
manufacturer/tradename	Ascentis®
packaging	1 ea of
parameter	60 °C temp. range
technique(s)	HPLC: suitable
L × I.D.	15 cm × 4.6 mm
surface area	90 m2/g
impurities	<5 ppm metals
matrix	Fused-Core particle platform
matrix active group	PFP (pentafluorophenyl) phase
particle size	5 μm
pore size	90 Å pore size
operating pH	2-8
application(s)	food and beverages
separation technique	reversed phase
Quality Level	100

FAQs

Question: What stationary phase is used in the Ascentis Express F5 column?

Answer:It uses a pentafluorophenyl, or F5/PFP, bonded phase.

Question: What are the column dimensions and particle size?

Answer:The column is 15 cm × 4.6 mm and contains 5 µm superficially porous particles.

Question: What is the pore size?

Answer:The Fused-Core particles have 90 Å pores.

Application Note

Ascentis® Express F5, 5 µm HPLC Column is used for reversed-phase separations that benefit from pentafluorophenyl selectivity rather than conventional C18 retention alone. This analytical column measures 15 cm × 4.6 mm and uses 5 µm superficially porous Fused-Core® particles with 90 Å pores. The F5 stationary phase provides multiple interaction mechanisms, including hydrophobic, dipole, pi-pi and shape-selective contributions, which can improve resolution of aromatic, halogenated, positional-isomer and other structurally similar compounds. The column is endcapped, classified as USP L43 and specified for operation over approximately pH 2–8. It supports pressures up to about 600 bar and temperatures up to 60°C. The superficially porous particle design provides high efficiency with manageable backpressure on conventional HPLC systems.

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