Description
General description
In the presence of a chaotropic salt, RNA binds selectively to special glass fibers prepacked in the High Pure Spin Filter Tube. Bound RNA is purified in a series of rapid wash-and-spin steps to remove contaminating salts, proteins, and other cellular impurities, and then eluted using a low-salt solution. By lowering the concentration of Binding Enhancer during the binding step in the two-column protocol, the small RNA-containing miRNA passes the first column unbound. When the concentration of Binding Enhancer is increased, the small RNA fraction can be bound to a second High Pure Spin Filter Tube.
Low to medium throughput miRNA isolation.
The High Pure miRNA Isolation Kit purifies and enriches small RNAs, such as microRNA (miRNA) from animal cells and tissue samples (including formalin-fixed, paraffin-embedded sections) or plant material. It can also be used to purify total RNA or to prepare samples enriched for small RNAs (Capacity: The High Pure Spin Filter Tubes hold up to 700 μL sample volume.
Application
The High Pure miRNA Isolation kit rapidly purifies RNA that is suitable for direct use in many downstream applications:
Northern blotting
cDNA synthesis
Primer extension
miRNA array hybridization
Relative quantification of miRNA with RT-PCR using, for example, the LightCycler® 480 System
Features and Benefits
Isolate DNA-free miRNA ideal for qualitative and quantitative reverse transcription. ideal for qualitative and quantitative reverse transcription.
Obtain excellent performance and linearity in RT-PCR.
Generate stable , highly pure miRNAs using a simple, efficient protocol.
Purify miRNA without using hazardous organic solvents. Avoid phenol/chloroform steps required by other suppliers′ miRNA purification kits.
Choose one flexible kit for all your miRNA purifications. Use the same kit to purify small RNAs from a variety of sample types.
Analysis Note
More than 60% recovery is obtained when 1 μg miRNA is added to106 K-562 cells and isolated with the one-column method, and more than 50% is recovered with the two-column method.
Other Notes
High Pure Technology and Silica Adsorption Kits
Tissue Lysis Buffer (for FFPE sections)
Proteinase K, recombinant (for FFPE sections)
Binding Buffer
Binding Enhancer
Wash Buffer
Elution Buffer
High Pure Filter Tubes
Collection Tubes
For life science research only. Not for use in diagnostic procedures.
Legal Information
LightCycler is a registered trademark of Roche
