Merck, 524742-10U, Phosphodiesterase 3A, Active, Human, Recombinant, S. frugiperda

General description
Full-length, recombinant, human phosphodiesterdase 3A (PDE3A) expressed in S. frugiperda insect cells using a baculovirus expression system. PDE3A is expressed primarily in cardiac and vascular myocytes and platelets. Supplied as a partially purified preparation of catalytically active enzyme useful for inhibitor and activator assays.
Physical form
In 150 mM NaCl, 10 mM Tris, 0.1% NP-40, pH 8.3.
Preparation Note
Following reconstitution, aliquot and freeze (-70°C).
Other Notes
One unit is defined as the amount of enzyme that is required to convert 1 pmol cAMP to AMP per min at pH 7.5, 30°C.
Shakur, Y., et al. 2000. J. Biol. Chem.275, 38749.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)