Merck, 528208-50UG, Plasminogen Activator Inhibitor-1, Mutant, Human, Recombinant

General description
Highly purified preparation of an altered form of human PAI-1 containing four mutated amino acids. Mutant PAI-1 is virtually unable to go latent and is stable at elevated temperature and pH for extended periods of time (t½ = 145 hours at 37°C, pH 7.4). Inhibits uPA (Ki = 5.1 x 10⁶ M⁻¹ sec⁻¹) and tPA (Ki = 7.9 x 10⁵ M⁻¹ sec⁻¹).
Biochem/physiol Actions
Cell permeable: no
Primary Target
Plasminogen Activator
Product does not compete with ATP.
Reversible: no
Target Ki: 5.1 x 106 M-1 sec-1, 7.9 x 105 M-1 sec-1 against uPA and tPA, respectively
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 100 mM NaCl, 50 mM sodium phosphate buffer, 1 mM EDTA, pH 6.6.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Berkenpas, M.B., et al. 1995. EMBO J. 14, 2969.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)