Merck, 528215-100UG, Anti-PAI-1 Mouse mAb (MA-33B8)

General description
Purified mouse monoclonal antibody. Recognizes the ~50 kDa PAI-1 protein.
Recognizes the ~50 kDa PAI-1 protein.
This Anti-PAI-1 Mouse mAb (MA-33B8) is validated for use in Immunoblotting, Neutralization Studies for the detection of PAI-1.
Immunogen
Human
human tPA-PAI-1 complex
Application
Immunoblotting (10 µg/ml)
Neutralization Studies (see application references)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 100 mM NaCl, 50 mM sodium phosphate buffer, pH 7.4.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Montes, R., et al. 2000. Thromb. Haemost.84, 65.
Verhamme, I., et al. 1999. J. Biol. Chem.274, 17511.
Debrock, S., and Declerck, P.J. 1997. Biochim. Biophys. Acta1337, 257.
Rapidly inactivates PAI-1 (Plasminogen Activator Inhibitor-1) to the latent form. Like the native latent form, PAI-1 inactivated by antibody was protected from cleavage by elastase. Also shown to reduce renal fibrin deposits in a rabbit model of endotoxemia. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)