Merck, 538540, MMP-7, Proenzyme, Human, Recombinant, E. coli

General description
Recombinant, human pro-MMP-7 expressed in E. coli. MMP-7 is believed to be a key factor in the regulation of metastasis. Overexpression of MMP-7 is observed in colon cancer. Activatable by organomercurials (APMA; Cat. No. 164610).
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 150 mM NaCl, 25 mM Tris-HCl, 5 mM CaCl₂, 0.02% NaN₃, 0.01% Brij® 35 Detergent, pH 7.5.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C). Freeze-thaw cycles may accelerate the activation process.
Other Notes
Girolamo, N. D., et al. 2001. Invest. Ophtal. Visual. Sci.42, 1963.
Kihira, Y., et al. 1996. Urol. Oncol. 2, 20.
Woessner, J.F. 1995. Methods Enzymol. 248, 485.
Crabbe, T., et al. 1992. Biochemistry 31, 8500.
One unit is defined as the amount of enzyme that will digest 1.0 µg AZOCOLL Substrate (Cat. No. 194933), in the presence of 500 μM p-aminophenylmercuric acetate (APMA; Cat. No. 164610) per minute at 37°C, pH 7.5.
Legal Information
Brij is a registered trademark of Croda International PLC
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)