Merck, 553009-10T, PhosphoDetect Anti-c-Raf (pTyr³⁴⁰, Tyr³⁴¹) Rabbit pAb

General description
Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~74 KDa c-Raf protein phosphorylated on Tyr³⁴⁰/³⁴¹.
Recognizes c-Raf phosphorylated at Tyr³⁴⁰ and Tyr³⁴¹ in HEK293 cells overexpressing c-Raf.
This PhosphoDetect Anti-c-Raf (pTyr³⁴⁰, Tyr³⁴¹) Rabbit pAb is validated for use in WB, ICC for the detection of c-Raf (pTyr³⁴⁰, Tyr³⁴¹).
Immunogen
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr³⁴⁰ and Tyr³⁴¹, phosphorylation sites of human c-Raf (Raf-1)
Application
Immunoblotting (1:1000)
Immunocytochemistry (see comments)
Physical form
In phosphate buffer (without Mg²⁺ and Ca²⁺), 1 mg/ml BSA, 50% glycerol, pH 7.3 (+/- 0.1).
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
HEK293 cells over expressing c-Raf, phosphorylated in vitro by Src.
Other Notes
The immunogen sequence is 100% conserved in mouse and rat, but cross-reactivity has not been tested. This antibody has also been reported to work for immunocytochemistry. Variables associated with assay conditions will dictate the proper working dilution.
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Yip-Schneider, M.T., et al. 2000. Biochem. J.351, 151.
Mason, C.S. et al. 1999. EMBO J.18, 2137.
Bonner, T.I. et al. 1985. Mol. Cell. Biol.5, 1400.
Moelling, K. et al. 1984. Nature312, 558.
Rapp, U.R. et al. 1983. Proc. Natl. Acad. Sci. USA80, 4218.
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Toxicity: Standard Handling (A)