Merck, 553504-10KU, RAD51, His•Tag®, Pneumocystis carinii, Recombinant, E. coli

General description
Recombinant, Pneumocystis carinii RAD51 fused at the N-terminus to a His•Tag sequence and expressed in E. coli. The Pneumocystis carinii RAD51 is equivalent to bacterial RecA and the homologue of yeast RAD51. It plays a pivotal role in the homologous recombination pathway for DNA double-strand break (DSB) repair. RAD51 has been shown to colocalize with tumor suppressor proteins BRCA1 (Cat. No. 203659) and BRCA2 in DNA-damage-induced foci and RAD51 is known to interact directly with BRCA2.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 0.2 mM EDTA, 20% glycerol, pH 8.0.
Other Notes
One unit equals 1 ng of purified protein. 20 units are sufficient for a DNA-protein assay and 100 units are sufficient for a protein-protein interaction assay.
West, S.C. 2003. Nat. Rev. Mol. Cell Biol.4, 435.
Jasin, M. 2002. Oncogene21, 8981.
Symington, L.S., et al. 2002. Microbiol. Mol. Biol. Rev.66, 630.
Daboussi, F., et al. 2002. Cell. Signal.14, 969.
Liu, Y. and West, S.C. 2002. Breast Cancer Res.4, 9.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)