Merck, 559309-10T, PhosphoDetect Anti-SAPK/JNK (pThr¹⁸³, Tyr¹⁸⁵) Rabbit pAb

General description
Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~49 kDa JNK1/SAPK1 protein and the ~55 kDa JNK2/SAPK2 protein phosphorylated at Thr¹⁸³ and Tyr¹⁸⁵.
Recognizes the ~49 kDa SAPK1 protein and the ~55 kDa SAPK2 protein phosphorylated at Thr¹⁸³ and Tyr¹⁸⁵.Antibody Target Gene Symbol: MAPK8, MAPK9
Target Synonym: AI849689, C-JUN N-TERMINAL KINASE1, JNK, JNK1, JNK1 PROTEIN KINASE, JNK1A2, JNK21B1/2, p46JNK1, p46JNK1 ALPHA, PRKM8, Sapk gamma, SAPK P46, SAPK1, SAPK1/JNK, STRESS-ACTIVATED PROTEIN KINASE-LIKE KINASE, AI851083, Jnk p54, Jnk p55, JNK-55, JNK2, JNK2A, Jnk2a2, JNK2ALPHA, JNK2B, JNK2BETA, JUNKINASE, p54a, p54aSAPK, PRKM9, SAPK, SAPK ALPHA, SAPK ALPHA 1
Entrez Gene Name: mitogen-activated protein kinase 8
Hu Entrez ID: 5599 ;-; 5601
Mu Entrez ID: 26419 ;-; 26420
Rat Entrez ID: 116554 ;-; 50658

This PhosphoDetect Anti-SAPK/JNK (pThr¹⁸³, Tyr¹⁸⁵) Rabbit pAb is validated for use in WB, IHC for the detection of SAPK/JNK (pThr¹⁸³, Tyr¹⁸⁵).
Immunogen
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸³ and Tyr¹⁸⁵ phosphorylation sites of human SAPK/JNK
Application
Immunoblotting (1:1000)
Immunohistochemistry (see comments)
Physical form
In Dulbecco′s PBS (without Mg²⁺ and Ca²⁺), 50% glycerol, 1 mg/ml BSA, pH 7.3.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Fanger, G.R., et al. 1999. Histol. Histopathol.14, 887.
Gabai, V.L., et al. 1998. FEBS Lett.438, 1.
Specific for the dually phosphorylated form of JNK/SAPK containing a phosphate on threonine 183 and tyrosine 185. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)