Merck, 565324-U, Ascentis® RP-Amide HPLC Column

Name: Ascentis® RP-Amide HPLC Column
Brand: Merck
SKU: 565324-U

Size:1 ea, RP-Amide Reversed-Phase Chromatography Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

Catalog Number: 565324-U

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Category

Reversed-Phase HPLC Columns

Pack Size1 ea

TagsHPLC Columns Analytical Chemistry Analytical Chromatography

CollectionsMerck Analytical Chromatography Products & Lab Supplies Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

Ascentis RP-Amide is a new generation ultra low bleed, embedded polar group (EPG) phase that provides orthogonal selectivity and increased resolution for HPLC and LC-MS analysis of polar compounds. The Ascentis RP-Amide is the first choice in embedded polar group HPLC phases.
Application

Preparation of mixed-mode stationary phase for separation of peptides and proteins in high-performance liquid chromatography: Highlights the application of Ascentis® RP-Amide HPLC column in the development of mixed-mode stationary phases, further demonstrating its versatility in handling diverse biochemical compounds and its pivotal role in advancing pharmaceutical and proteomic research (Alharthi et al., 2022).

On-line hyphenation of solid-phase extraction to chromatographic separation of sulfonamides with fused-core columns in sequential injection chromatography: Showcases the efficacy of Ascentis® RP-Amide HPLC columns in environmental analysis, specifically for the detection of contaminants in complex matrices. This study underscores the column′s robustness and high-resolution capabilities, making it invaluable in environmental laboratories (Batista et al., 2015).

Determination of zearalenone and its metabolites in endometrial cancer by coupled separation techniques: Demonstrates the critical role of Ascentis® RP-Amide HPLC columns in clinical research, particularly in the sensitive and accurate measurement of biomarkers in cancer diagnostics. The column′s superior retention for polar compounds proves essential for precise biomedical analyses (Gadzała-Kopciuch et al., 2011).

Features and Benefits
Excellent retention and peak shape for polar compounds
100% aqueous compatibility
Ultra low bleed, LC-MS compatible
Unique selectivity

Other Notes
Discover LiChropur reagents ideal for HPLC or LC-MS analysis
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Specifications

General

UNSPSC Code	41115700
eCl@ss	32110501
Web Name Suffix Override	L × I.D. 15 cm × 4.6 mm, HPLC Column
Web Name Override	Ascentis® RP-Amide (5 µm) HPLC Columns
NACRES	SB.52
material	stainless steel column
agency	suitable for USP L60
product line	Ascentis®
feature	endcapped
manufacturer/tradename	Ascentis®
packaging	1 ea of
extent of labeling	19.5% Carbon loading
parameter	≤70 °C temp. range
technique(s)	HPLC: suitable
L × I.D.	15 cm × 4.6 mm
surface area	450 m2/g
surface coverage	2.7 μmol/m2
impurities	<5 ppm metals
matrix	fully porous particle
matrix active group	amide, alkyl phase
particle size	5 μm
pore size	100 Å
operating pH range	2-8
application(s)	food and beverages
separation technique	reversed phase
Quality Level	100

FAQs

Question: What are the dimensions of this Ascentis RP-Amide column?

Answer:The column is 150 mm long with a 4.6 mm internal diameter.

Question: What particle and pore sizes are used?

Answer:It contains 5 µm particles with 100 Å pores.

Question: What is distinctive about the RP-Amide stationary phase?

Answer:It uses an embedded polar amide group to provide alternative reversed-phase selectivity and strong performance for polar analytes.

Application Note

Ascentis® RP-Amide HPLC Column is used for reversed-phase separation of small molecules, particularly polar analytes that can be difficult to retain or resolve on conventional C18 phases. The stationary phase uses high-purity spherical fully porous silica with a 100 Å pore size and an embedded amide polar group in an alkyl bonded phase. This configuration is 150 mm long by 4.6 mm internal diameter and contains 5 µm particles. The phase is endcapped, has approximately 19.5% carbon loading and operates over a stated pH range of 2–8. Its embedded polar-group chemistry provides alternative selectivity, improved retention and peak shape for polar compounds, compatibility with fully aqueous mobile phases and low bleed for LC-MS work. The stainless-steel column is classified as USP L60 and is suitable for conventional analytical HPLC and LC-MS workflows.

Ordering

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