Merck, 565758-1MG, β-Secretase Substrate IV, Fluorogenic

General description
A highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate (excitation max.: ~350 nm; emission max.: ~490), based on the GVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein. Hydrolysis of the substrate at Leu-Asp bond by β-secretase results in fluorescence enhancement. Reported Kcat/km values (min⁻¹/µM) for pro-mempasin 2 and its proteolytically activated species Leu²⁸p-mempasin 2 are 0.044 and 0.040, respectively (at pH 4.5, 37°C).
Biochem/physiol Actions
Product does not compete with ATP.
Reversible: no
Cell permeable: no
Primary Target
β-secretase substrate
Packaging
Packaged under inert gas
Physical form
Supplied as a trifluoroacetate salt.
Preparation Note
Following reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 1 month at -20°C.
Other Notes
Ermolieff, J., et al. 2000. Biochemistry 39, 12450.
H-Arg-Glu(EDANS)Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(DABCYL)Arg-OH
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)