Merck, 567726-10MG, D-erythro-Sphingosine, Free Base, High Purity

General description
Highly purified preparation of bovine brain sphingosine containing >99% of the erythro isomer. A potent, cell-permeable, reversible, and selective inhibitor of protein kinase C (PKC; IC₅₀ = 2.8 µM) and insulin receptor tyrosine kinase. PKC inhibition is competitive with respect to diacylglycerol, phorbol dibutyrate, and Ca²⁺. Does not affect the activity of myosin light chain kinase and protein kinase A. Induces apoptosis in human leukemia HL-60 cells. Note: This product is not to be used for animal treatment, in vivo research or in any other contact procedure with livestock.
Biochem/physiol Actions
Cell permeable: no
Primary Target
PKC
Product does not compete with ATP.
Reversible: no
Target IC₅₀: 2.8 µM against PKC
Preparation Note
Following reconstitution, aliquot, purge with N₂ gas, and freeze (-20°C). Stock solutions are stable for up to 6 months under inert atmosphere at -20°C.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Irritant (B)