Merck, 568523-U, Discovery® HS C18 HPLC Column

Discovery® HS C18 HPLC Column is used for high-performance reversed-phase separation of hydrophobic and moderately polar small molecules. The stationary phase consists of high-purity spherical fully porous silica with a C18 bonded phase, 120 Å pores and 5 µm particle size. This configuration measures 250 mm in length by 4.6 mm internal diameter. The phase is endcapped, has approximately 20% carbon loading and is classified as USP L1. Its high surface area and hydrophobic C18 coverage provide strong reversed-phase retention, while low metal content and controlled surface chemistry support reproducible HPLC and LC-MS work. The stated operating pH range is 2–8, with a temperature limit of 70°C and pressure rating up to 400 bar. The conventional analytical dimensions make the column suitable for routine methods that require robust C18 selectivity.