Description
General description
HybridSPE-PLus is the next generation of HybridSPE-Phospholipid technology. The first of its kind, HybridSPE-Phospholipid technology was first introduced in 2008. It fuses the simple, standardized methodology of traditional protein precipitation with the specificity of solid phase extraction (SPE) for the simultaneous removal of proteins and phospholipids from biological samples prior to LC-MS analysis. Unlike other phospholipid removal products that use a hydrophobic retention mechanism to remove phsopholipids from biological samples, HybridSPE-Phospholipid technology uses a unique Lewis acid/base retention mechanism that allows it to separate phospholipids from even very hydrophobic analytes, such as vitamins, which are often retained along with phospholipids on competitive products.
Our HybridSPE-PLus products offer the same time-proven chemistry of our traditional HybridSPE-Phospholipid products coupled with manufacturing enhancements that stem from years of production experience. These manufacturing enhancements have allowed us to increase flow consistency and reduce sample hold up volumes, further improving analyte recoveries and assay reproducibility. We invite customers to take advantage of the newest technology; HybridSPE-PLus.
Application
Development of a High-Throughput Analytical Method for Antimicrobials in Wastewater Using an Automated Pipetting and Solid-Phase Extraction System: This study highlights the utilization of HybridSPE®-PLus 96-Well Plates for environmental analyte testing in wastewater. The method incorporates high-throughput sample preparation and solid phase extraction to efficiently process large sample volumes, proving essential for monitoring pharmaceutical pollutants and ensuring environmental safety (Azuma et al., 2024).
High-throughput DNA extraction and cost-effective miniaturized metagenome and amplicon library preparation of soil samples for DNA sequencing: This research utilizes HybridSPE®-PLus 96-Well Plates to streamline the preparation of soil sample libraries for high-throughput sequencing. The method emphasizes cost-efficiency and rapid processing, suitable for extensive environmental and biological studies, reflecting the plate′s versatility in sample preparation (Jensen et al., 2024).
Solvent-free parallel artificial liquid membrane extraction for drugs of abuse in plasma samples using LC-MS/MS: The HybridSPE®-PLus 96-Well Plate is employed for innovative LC-MS/MS sample cleanup in the detection of drugs in plasma. This application underscores the plate′s capacity for enhancing analytical performance in forensic and clinical toxicology by providing a robust, reproducible method for drug testing (Fabris et al., 2024).
High-throughput sequencing of insect specimens with sub-optimal DNA preservation using a practical, plate-based Illumina-compatible Tn5 transposase library preparation method: In this study, HybridSPE®-PLus 96-Well Plates facilitate high-throughput DNA extraction and sequencing of insect specimens. This highlights the plate′s crucial role in genomic research, particularly in the study of biodiversity and conservation biology, where rapid and efficient DNA analysis is required (Cobb et al., 2024).
Simultaneous determination of twelve neonicotinoids and six metabolites in human urine with isotope-dilution UPLC-Q-Orbitrap HRMS: This publication details the use of HybridSPE®-PLus 96-Well Plates in a sophisticated chromatographic setup for the quantitative analysis of pesticides in biological samples. The method demonstrates the plates′ effectiveness in environmental and health-related studies, ensuring precise measurement and monitoring of hazardous substances (Zhang et al., 2024).
Features and Benefits
Simple 2-3 step standard methodology analogous to traditional protein precipitation provides simultaneous removal of both proteins and phospholipids
Unique Lewis acid/base retention mechanism effectively removes phospholipids from both hydrophilic and hydrophobic analytes of interest (unlike competitive chemistries) eliminating phospholipid-induced ion suppression
Highly reproducible, consistent product performance
Provides cleaner samples for longer column life and less instrument downtime
Available in 96-well and 1 mL cartridge dimensions
Legal Information
HybridSPE is a registered trademark of Merck KGaA, Darmstadt, Germany
