Merck, 577801-100UG, Anti-Tau Mouse mAb (TAU-5)

General description
Protein G purified mouse monoclonal antibody. Recognizes the ~45-68 kDa phosphorylated and unphosphorylated forms of tau.
Recognizes the ~45-68 kDa Tau protein.
This Anti-Tau Mouse mAb (TAU-5) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of Tau.
Immunogen
Bovine
Epitope: within the central region
purified bovine microtubule-associated proteins
Application
Frozen Sections (1-2 µg/ml)
Immunoblotting (1 µg/ml)
Immunoprecipitation (10 µg per 200-500 µg cell lysate)
Paraffin Sections (1-2 µg/ml, heat pre-treatment required)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In PBS.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C). Do not store diluted antibody without carrier protein if the concentration is Proc. Natl. Acad. Sci. USA99, 1140.
Rapoport, M., eta al. 2002. Proc. Natl. Acad. Sci. USA99, 6364.
When used for formalin/paraffin embedded sections, staining is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temperature for 20 min prior to antibody incubation. Alternate splicing of tau mRNA and differential phosphorylation contribute to the heterogeneity of tau. Does not cross-react with other MAPS of tubulin. Recognizes both native and phosphorylated forms of tau. Variables associated with assay conditions will dictate proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)