Description
General description
A ready-to-use stable, neutral form of the popular disulfide-reducing agent TCEP, Hydrochloride (Cat. No. 580560). Reconstitute each vial with 1 ml H2O to obtain a 500 mM solution.
A ready-to-use, stable, neutral form of TCEP hydrochloride (Cat. No. 580560), a disulfide reducing agent.
Application
Förster Resonance Energy Transfer Assay for Investigating the Reactivity of Thioesters in Biochemistry and Native Chemical Ligation.: This study utilizes TCEP in a FRET assay to explore thioester reactivity, providing insights into biochemical reactions and peptide synthesis applications (Gless et al., 2023).
Two-Dimensional Capillary Zone Electrophoresis-Mass Spectrometry: Intact mAb Charge Variant Separation Followed by Peptide Level Analysis Using In-Capillary Digestion.: TCEP is employed for in-capillary digestion to enhance protein separation and analysis, improving the characterization of monoclonal antibodies (Schlecht et al., 2023).
Silica Nanoparticle-Loaded Thermoresponsive Block Copolymer Vesicles: A New Post-Polymerization Encapsulation Strategy and Thermally Triggered Release.: TCEP is used in the synthesis of block copolymer vesicles, facilitating the encapsulation and controlled release of nanoparticles for biomedical applications (Czajka et al., 2022).
Thiol Catalysis of Selenosulfide Bond Cleavage by a Triarylphosphine.: This research demonstrates the use of TCEP in catalyzing selenosulfide bond cleavage, contributing to the understanding of thiol-based reduction mechanisms (Firstova et al., 2022).
A Novel PNGase Rc for Improved Protein N-Deglycosylation in Bioanalytics and Hydrogen-Deuterium Exchange Coupled With Mass Spectrometry Epitope Mapping Under Challenging Conditions.: TCEP is highlighted for its role in improving protein deglycosylation and mass spectrometry analysis, enhancing bioanalytical techniques (Gramlich et al., 2022).
Packaging
Packaged under inert gas
Preparation Note
Following reconstitution, aliquot, flush with an inert gas, and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Reconstitute with 1 ml water to obtain a 500 mM solution.
Other Notes
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Getz, E.B., et al. 1999. Anal. Biochem.273, 73.
Han, J.C. and Han, G.Y. 1994. Anal. Biochem.220, 5.
Burns, J.A., et al. 1991. J. Org. Chem.56, 2648.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Corrosive (L)
